The Patch-seq recording technique is a powerful approach ( Cadwell et al., 2017 Cadwell et al., 2015 Fuzik et al., 2015 van den Hurk et al., 2018) that can provide morphology (M), electrophysiology (E), and transcriptome (T) data from single neuron, that is triple modality MET data. These data are an important tool toward establishing ‘correspondence’ of these properties through analysis. This technique is a modification of the well-established slice patch-clamp electrophysiology approach, where intrinsic properties are recorded from neurons in acute brain slices while simultaneously filling the neuron with biocytin. #Baker aps universal bu2010 reset softwareįollowing fixation, the biocytin is reacted with DAB as chromogen to generate a dark precipitate that fills the neuron, enabling imaging and a digital morphological reconstruction.
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